产品货号：
Z37199
中文名称：
PKM2/PKM抗体
英文名称：
Anti-PKM2/PKM Antibody (Clone#11I4C3)
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-PKM2/PKM Antibody (Clone#11I4C3) |
| 指标别名 | CTHBP;OIP 3;OIP3;Opa interacting protein 3;p58;PK2;PK3;PKM;PKM2;PK-M2;PKM2 pyruvate kinase;muscle;Pyruvate kinase 2/3;Pyruvate kinase isozymes M1/M2;Pyruvate kinase muscle isozyme;TCB;THBP1;Tumor M2 PK |
| 克隆性 | Monoclonal |
| 检验物种 | human |
| 应用范围 | WB,IHC,ICC/IF,FCM |
| 基因名称 | PKM |
| 克隆号 | 11I4C3 |
| 抗体来源 | Mouse |
| 抗体类型 | IgG2b |
| 免疫原 | A synthetic peptide corresponding to a sequence at the N-terminus of human PKM2(475-500aa KDPVQEAWAEDVDLRVNFAMNVGKAR),different from the related mouse sequence by five amino acids,and from the related rat sequence by four amino acids. |
| 实际分子量 | 58KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | PKM (Pyruvate Kinase,Muscle),also known as PK3 or PKM2,is an enzyme that in humans is encoded by the PKM gene.The activity of pyruvate kinase subtype M2 is increased by fructose 1,6-bisphosphate (Fru-1,6-P2).By in situ hybridization,Popescu and Cheng (1990) mapped the THBP1 gene to 15q24-q25.Ashizawa et al.(1991) manipulated the intracellular Fru-1,6-P2 concentration in several mammalian cell lines,including human,by varying the glucose concentration in the media.Using a novel proteomic screen for phosphotyrosine-binding proteins,Christofk et al.(2008) observed that PKM2 binds directly and selectively to tyrosine-phosphorylated peptides. |
| Uniprot ID | P14618 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (ZN1928) for Western blot,and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (ZN1866) for IHC(P) and ICC. |
| 基因名全称 | pyruvate kinase,muscle |
| 蛋白名全称 | Pyruvate kinase PKM |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
相关搜索：PKM2/PKM抗体，PKM2抗体，PKM单抗，Anti-PKM2/PKM Antibody (Clone#11I4C3)